產(chǎn)品描述: | Methylene Blue (Basic Blue 9, Tetramethylthionine chloride, methylthioninium chloride, CI-52015)在色素內(nèi)鏡檢查中被作用染料。它能抑制tau蛋白絲的形成,IC50為1.9μM,同時(shí)還抑制可溶性鳥苷環(huán)化酶 |
靶點(diǎn): |
guanylate cyclase; Tau aggregation:1.9 μM;Guanylatecyclase;MicrotubuleAssociated;NOSynthase;Parasite;MonoamineOxidase |
體外研究: |
Methylene Blue可能抑制NO合成酶或通過產(chǎn)生超氧陰離子,直接使一氧化氮失活。除了干擾一氧化氮換鳥苷酸途徑,Methylene Blue直接激活鈣離子依賴性的鉀通道來增強(qiáng)去甲腎上腺素從細(xì)胞內(nèi)的釋放 |
體內(nèi)研究: |
Methylene Blue抑制鳥苷酸環(huán)化酶,減少C-GMP和血管平滑松弛。當(dāng)進(jìn)行靜脈注射時(shí),亞甲藍(lán)在甲狀旁腺積累,從而在外科手術(shù)中借以其顏色來判斷這些內(nèi)部結(jié)構(gòu)。在嚙齒類的藥動(dòng)學(xué)實(shí)驗(yàn)揭示,血管內(nèi)進(jìn)行MB給藥將導(dǎo)致MB在中樞神經(jīng)細(xì)胞中迅速?gòu)V泛的積累 |
細(xì)胞實(shí)驗(yàn): |
Cell lines: 未成熟的成神經(jīng)細(xì)胞 Concentrations: 10 μM Incubation Time: 2 h Method: 從新生大鼠的室管膜下層分離出來的未成熟的成神經(jīng)細(xì)胞,在單細(xì)胞培養(yǎng)模型中,分化成γ氨基丁酸所介導(dǎo)神經(jīng)元,用此神經(jīng)元細(xì)胞用于后續(xù)實(shí)驗(yàn)。在體外實(shí)驗(yàn)中,第6天,用MB對(duì)細(xì)胞培養(yǎng)物進(jìn)行處理2h,處理完48小時(shí)后,檢測(cè)細(xì)胞樹突分枝結(jié)構(gòu)及生存情況 |
動(dòng)物實(shí)驗(yàn): |
Animal Models: Sprague- Dawley雄性大鼠 Dosages: 5 and 50 mg/kg Administration: 血管內(nèi)注射 |
參考文獻(xiàn): |
1. Vutskits L, et al. Adverse effects of methylene blue on the central nervous system. Anesthesiology. 108(4):684-692. 2. Hochgr鋐e K, et al. Preventive methylene blue treatment preserves cognition in mice expressing full-length pro-aggregant human Tau. Acta Neuropathol Commun. 2015, 3:25. 3. Masaki E, et al. Methylene blue, a soluble guanylyl cyclase inhibitor, reduces the sevoflurane minimum alveolar anesthetic concentration and decreases the brain cyclic guanosine monophosphate content in rats. Anesth Analg. 1999, 89(2):484-489. 4. Ginimuge PR, et al. Methylene blue: revisited. J Anaesthesiol Clin Pharmacol. 2010, 26(4):517-520. |
溶解性: |
Soluble in DMSO、Ethanol、H2O |
保存條件: |
-20℃ |
配置溶液濃度參考: |
|
1mg |
5mg |
10mg |
1 mM |
3.126 ml |
15.632 ml |
31.265 ml |
5 mM |
0.625 ml |
3.126 ml |
6.253 ml |
10 mM |
0.313 ml |
1.563 ml |
3.126 ml |
50 mM |
0.063 ml |
0.313 ml |
0.625 ml |
|
注意: |
部分產(chǎn)品我司僅能提供部分信息,我司不保證所提供信息的權(quán)威性,僅供客戶參考交流研究之用。 |